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KHK (Ketohexokinase) (12H10) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0140
产品名称
KHK (Ketohexokinase) (12H10) Mouse Monoclonal antibody
类别
常规抗体
基因名称
KHK
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human KHK (NP_000212) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:50, IF 1:100, FCM 1:100
预测分子量
32.5kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody (BD-PE0140), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0140, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0140, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0140, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0140, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0140, Dilution 1:50)

Anti-KHK mouse monoclonal antibody (BD-PE0140) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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