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IDS (Iduronate 2 sulfatase) (11P3) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0124
产品名称
IDS (Iduronate 2 sulfatase) (11P3) Mouse Monoclonal antibody
类别
常规抗体
基因名称
Iduronate 2 sulfatase
推荐应用
WB
反应种属
Human,Mouse
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human IDS(NP_000193) produced in HEK293T cell.
稀释度
WB 1:500~2000, IHC 1:150, IF 1:100, FCM 1:100
预测分子量
59.2kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2a
背景介绍
Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IDS antibody (BD-PE0124), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-IDS antibody (BD-PE0124), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Jurkat cells, using anti-IDS antibody (BD-PE0124), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (10ug) from a mouse cell line by using anti-IDS monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0124)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0124)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDS monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0124)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0124)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0124)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0124)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IDS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0124)

Anti-IDS mouse monoclonal antibody (BD-PE0124) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDS .

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-IDS monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDS. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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