Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ICAM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE0123) (1:150)

HEK293T cells transfected with either overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-ICAM1 antibody (BD-PE0123), and then analyzed by flow cytometry (1:100).

Flow cytometric Analysis of living HUVEC cells, using anti-ICAM1 antibody (BD-PE0123), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Western blot analysis of extracts (35ug) from A549 cells (-), HUVEC cells (-) and A549 cells treated with 20ng/ml TNF-a for 24h (+), using anti-ICAM1 monoclonal antibody (1:100).

Western blot analysis of extracts (35ug) from 2 different cell lines and human spleen tissue by using anti-ICAM1 monoclonal antibody (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ICAM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ICAM1 (Cat# BD-PE0123)(1:500).