BD-PE0119-HMBS (18R10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HMBS (18R10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0119

产品名称

HMBS (18R10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

HMBS

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human HMBS (NP_000181) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-HMBS 44 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (BD-PE0119); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Weak nuclear staining is seen on tumor cells.

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Western blot analysis of HT29 cell lysate (35ug) by using anti-HMBS monoclonal antibody.

Western blot analysis of K562 cell lysate (35ug) by using anti-HMBS monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-HMBSmouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Western blot analysis of KM12 cell lysate (35ug) by using anti-HMBS monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human tonsil using anti-HMBS 44 mouse monoclonal antibody at 1:400 with Polink2 Broad HRP DAB detection kit (BD-PE0119); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen on tonsil germinal and non-germinal centers. Some weak nuclear stain was also observed.

Immunofluorescent staining of HeLa cells using anti-HMBS mouse monoclonal antibody (BD-PE0119, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-HMBS mouse monoclonal antibody. (BD-PE0119; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)