BD-PE0115-HK2 (Hexokinase II) (5X15) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HK2 (Hexokinase II) (5X15) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0115

产品名称

HK2 (Hexokinase II) (5X15) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Hexokinase II

推荐应用

反应种属

Human,Dog,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.

稀释度

WB 1:1000, IF 1:100, FCM 1:100

预测分子量

102.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways.This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

期货

2周

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (lane 1) , human HK2 plasmid (lane 2), mouse HK2 plasmid (lane 3) using anti-HK2 antibody BD-PE0115(1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2(Cat# BD-PE0115). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

HEK293T cells transfected with either pCMV6-ENTRY HK2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-HK2 mouse monoclonal (BD-PE0115), and then analyzed by flow cytometry.

Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (BD-PE0115).

Anti-HK2 mouse monoclonal antibody (BD-PE0115) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2 .

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.