BD-PE0105-NR3C1 (13U3) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NR3C1 (13U3) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE0105

产品名称

NR3C1 (13U3) Mouse Monoclonal antibody

类别

常规抗体

基因名称

NR3C1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 1-265 of human NR3C1(NP_000167) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150

预测分子量

85.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0105)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NR3C1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE0105)

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-NR3C1 monoclonal antibody (1:500).

Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-NR3C1 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR3C1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR3C1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.