RM1365-G6PC (10Y14) Rabbit Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

G6PC (10Y14) Rabbit Monoclonal antibody

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产品基本信息

产品货号

RM1365

产品名称

G6PC (10Y14) Rabbit Monoclonal antibody

类别

常规抗体

基因名称

glucose 6 phosphatase, catalytic subunit

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human G6PC (NP_000142). The exact sequence is proprietary.

稀释度

WB 1:2000, IHC 1:200-1:1000

预测分子量

40.5kDa

运输及保存条件

-20°C/1 year

宿主

Rabbit

同种型

IgG

背景介绍

Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of overexpressed lysates from HEK293T cells transfected with empty plasmid (lane 1, 15ug) and human G6PC recombinant protein (lane 2, 100ng) using anti-G6PC antibody RM1365(1:3000).

IHC staining of FFPE human duodenum tissue within the normal limits using anti-G6PC rabbit monoclonal antibody (RM1365) and Polink-2 HRP polymer detection kit . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

IHC staining of FFPE human kidney tissue within the normal limits using anti-G6PC rabbit monoclonal antibody (RM1365) and Polink-2 HRP polymer detection kit . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.

IHC staining of FFPE human liver tissue within the normal limits using anti-G6PC rabbit monoclonal antibody (RM1365) and Polink-2 HRP polymer detection kit . Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min. The brown stain indicates positive stain, blue is the counter stain.