BD-PE0088-FH (Fumarate Hydratase) (8T4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FH (Fumarate Hydratase) (8T4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0088

产品名称

FH (Fumarate Hydratase) (8T4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

推荐应用

反应种属

Human,Dog,Rat,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human FH (NP_000134) produced in HEK293T cell.

稀释度

WB 1:1000, IHC 1:50, IF 1:100, FCM 1:100

预测分子量

50.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0088)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-FH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0088)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-FH mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0088)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FH antibody (BD-PE0088), and then analyzed by flow cytometry.

Anti-FH mouse monoclonal antibody (BD-PE0088) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH(Cat# BD-PE0088). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.