BD-PE0085-FGFR2 (4Q17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FGFR2 (4Q17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0085

产品名称

FGFR2 (4Q17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FGFR2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human FGFR2(NP_000132) produced in HEK293T cell.

稀释度

WB 1:200 - 1:1000, IHC 1:150, IF 1:100, FCM 1:100

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in this gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis. Multiple alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

No Cross-Reactivity of anti-FGFR2 (BD-PE0085) with FGFR1, FGFR3 and FGFR4.HEK293T cells were transfected with the DDK (FLAG)-tagged DNAs of FGFR2 , FGFR1 , FGFR3 , FGFR4 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2 (BD-PE0085). (1:500) and anti-DDK (1:2000), respectively.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0085)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0085)

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-FGFR2 monoclonal antibody at 1:200.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FGFR2 antibody (BD-PE0085), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-FGFR2 antibody (BD-PE0085), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0085)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0085)

Anti-FGFR2 mouse monoclonal antibody (BD-PE0085) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FGFR2 .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FGFR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0085)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FGFR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.