BD-PE0071-ESR1 (Estrogen Receptor 1/ER) (3B9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ESR1 (Estrogen Receptor 1/ER) (3B9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0071

产品名称

ESR1 (Estrogen Receptor 1/ER) (3B9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ESR1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ESR1(NP_000116) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150

预测分子量

66kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

Figure from citation: Western Blot of ESR1 protein level by using anti-ESR1 antibody in human breast cancer tissues (T) and normal tissues (N).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ESR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0071)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ESR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESR1(Cat# BD-PE0071). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.