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EPHX1 (Epoxide Hydrolase 1) (6X11) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0067
产品名称
EPHX1 (Epoxide Hydrolase 1) (6X11) Mouse Monoclonal antibody
类别
常规抗体
基因名称
Epoxide hydrolase
推荐应用
WB
反应种属
Human,Rat,Monkey,Mouse
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 21-230 of human EPHX1 (NP_000111) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150
预测分子量
52.8kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
Epoxide hydrolase is a critical biotransformation enzyme that converts epoxides from the degradation of aromatic compounds to trans-dihydrodiols which can be conjugated and excreted from the body. Epoxide hydrolase functions in both the activation and detoxification of epoxides. Mutations in this gene cause preeclampsia, epoxide hydrolase deficiency or increased epoxide hydrolase activity. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2008]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0067)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHX1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0067)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-EPHX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0067)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX1(Cat# BD-PE0067). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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