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DLD (Lipoamide Dehydrogenase) (3B19) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE0057
产品名称
DLD (Lipoamide Dehydrogenase) (3B19) Mouse Monoclonal antibody
类别
常规抗体
基因名称
DLD
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human DLD(NP_000099) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100
预测分子量
50.1kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DLD antibody (BD-PE0057), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0057)

Anti-DLD mouse monoclonal antibody (BD-PE0057) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DLD .

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0057)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0057)

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-DLD monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-DLD monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DLD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DLD(Cat# BD-PE0057). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DLD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0057)

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