BD-PE0055-CYP17A1 (19J19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYP17A1 (19J19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE0055

产品名称

CYP17A1 (19J19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Cytochrome P450 17A1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYP17A1(NP_000093) peoduced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, FCM 1:100

预测分子量

57.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Flow cytometric Analysis of Jurkat cells, using anti-CYP17A1 antibody (BD-PE0055), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Hela cells, using anti-CYP17A1 antibody (BD-PE0055), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYP17A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0055)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYP17A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYP17A1(Cat# BD-PE0055). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.