Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE0022)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARG1 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARG1 antibody (BD-PE0022), and then analyzed by flow cytometry.

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ARG1 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARG1(Cat# BD-PE0022). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human ARG2 plasmid (hARG2), human ARG1 plasmid (hARG1) using anti-ARG1 antibody BD-PE0022(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)