产品名称
ALKB3 Rabbit Polyclonal Antibody
蛋白名称
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (EC 1.14.11.-) (Alkylated DNA repair protein alkB homolog 3) (DEPC-1) (Prostate cancer antigen 1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q96Q83
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q96Q83/entry
Mouse Swissprot No.
Q8K1E6
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q8K1E6
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q5XIC8
免疫原
Synthesized peptide derived from human protein . at AA range: 110-190
特异性
ALKB3 Polyclonal Antibody detects endogenous levels of protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008],
组织表达
Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
细胞定位
Nucleus . Cytoplasm . Colocalizes with ASCC2 and ASCC3 in nuclear foci when cells have been exposed to alkylating agents that cause DNA damage (PubMed:29144457). Predominantly localizes to the nucleus. .
功能
cofactor:Binds 1 Fe(2+) ion per subunit.,enzyme regulation:Activated by ascorbate.,function:Dioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.,similarity:Belongs to the alkB family.,tissue specificity:Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
