BD-PB5156-CALR (7O9) Mouse Monoclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

CALR (7O9) Mouse Monoclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB5156

产品名称

CALR (7O9) Mouse Monoclonal Antibody (Center)

别名

Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60, CALR (HGNC:1455), CRTC

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.

Human Swissprot No.

P27797

特异性

This CALR antibody is generated from a mouse immunized with a recombinant protein from human CALR.

稀释度

WB~~1:2000

预测分子量

48kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Mouse

同种型

IgG1,κ

背景介绍

细胞定位

Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen {ECO:0000250|UniProtKB:P28491}. Cytoplasmic vesicle, secretory vesicle, Cortical granule {ECO:0000250|UniProtKB:Q8K3H7}. Cytolytic granule. Note=Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). During oocyte maturation and after parthenogenetic activation accumulates in cortical granules. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (By similarity). In cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation (By similarity). {ECO:0000250|UniProtKB:P28491, ECO:0000250|UniProtKB:Q8K3H7, ECO:0000269|PubMed:8418194}

功能

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).

期货

现货

All lanes : Anti-CALR Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HeLa whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: K562 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lane 8: C6 whole cell lysate Lane 9: CHO whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-CALR Antibody (Center) at dilution Lane 1:HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: SH-SY5Y whole cell lysate Lane 6: K562 whole cell lysate Lane 7: NIH/3T3 whole cell lysate Lane 8: C6 whole cell lysate Lane 9: CHO whole cell lysat Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-CALR Antibody (Center) at dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.