BD-PB5140-BRAF V600E Rabbit Polyclonal Antibody(C-Term)-现货抗体产品库武汉枢密脑科学技术有限公司

BRAF V600E Rabbit Polyclonal Antibody(C-Term)

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产品基本信息

产品货号

BD-PB5140

产品名称

BRAF V600E Rabbit Polyclonal Antibody(C-Term)

别名

Serine/threonine-protein kinase B-raf, 2.7.11.1, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

P15056

特异性

This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 576-606 amino acids from human.

稀释度

IHC-P~~1:100~500;WB~~1:2000

预测分子量

84kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

组织表达

Brain and testis.

细胞定位

Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

功能

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).

期货

现货

BD-PB5140 staining BRAF in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-BRAF Antiboday(C-Term) at 1:2000 dilution Lane 1: Braf-600V lysate Lane 2: Braf-600E lysate Lane 3: Raji whole cell lysate Lane 4: Hela whole cell lysate Lane 5: A549 whole cell lysate Lane 6: T47D whole cell lysate Lane 7: MDA-MB-453 whole cell lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.