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TFF1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号
BD-PB5091
产品名称
TFF1 Rabbit Polyclonal Antibody (Center)
别名
Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, TFF1, BCEI, PS2
类别
常规抗体
推荐应用
WB
反应种属
Human
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
Human Swissprot No.
P04155
特异性
This TFF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 11-40 amino acids from the Central region of human TFF1.
稀释度
IF~~1:25;WB~~1:2000
预测分子量
9kDa
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
宿主
Rabbit
同种型
Rabbit IgG
背景介绍
Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
组织表达
Found in stomach, with highest levels in the upper gastric mucosal cells (at protein level). Detected in goblet cells of the small and large intestine and rectum, small submucosal glands in the esophagus, mucous acini of the sublingual gland, submucosal glands of the trachea, and epithelial cells lining the exocrine pancreatic ducts but not in the remainder of the pancreas (at protein level) Scattered expression is detected in the epithelial cells of the gallbladder and submucosal glands of the vagina, and weak expression is observed in the bronchial goblet cells of the pseudostratified epithelia in the respiratory system (at protein level). Detected in urine (at protein level). Strongly expressed in breast cancer but at low levels in normal mammary tissue. It is regulated by estrogen in MCF-7 cells. Strong expression found in normal gastric mucosa and in the regenerative tissues surrounding ulcerous lesions of gastrointestinal tract, but lower expression found in gastric cancer (at protein level).
细胞定位
Secreted
功能
Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.
期货
现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling TFF1 with BD-PB5091 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing secreted staining on MCF-7 cell line. The nuclear counter stain is DAPI (blue).

All lanes : Anti-TFF1 Antibody (Center) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: T47D whole cell lysate Lane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 9 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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