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PGM1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号
BD-PB5068
产品名称
PGM1 Rabbit Polyclonal Antibody (Center)
别名
Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
Human Swissprot No.
P36871
特异性
This PGM1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 251-282 amino acids from the Central region of human PGM1.
稀释度
WB~~1:2000;IHC-P~~1:100~500
预测分子量
61kDa
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
宿主
Rabbit
同种型
Rabbit IgG
背景介绍
This enzyme participates in both the breakdown and synthesis of glucose.
细胞定位
[Isoform 1]: Cytoplasm.
功能
This enzyme participates in both the breakdown and synthesis of glucose.
期货
现货

All lanes : Anti-PGM1 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: human liver lysate Lane 5: mouse brain lysate Lane 6: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

BD-PB5068 staining PGM1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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