BD-PB5035-CDK4 (8O17) Mouse Monoclonal Antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CDK4 (8O17) Mouse Monoclonal Antibody

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产品基本信息

产品货号

BD-PB5035

产品名称

CDK4 (8O17) Mouse Monoclonal Antibody

别名

Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, CDK4

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.

Human Swissprot No.

P11802

特异性

This CDK4 antibody is generated from a mouse immunized with a recombinant protein.

稀释度

IHC-P~~1:100~500;IHC~~1:300;WB~~1:2000

预测分子量

34kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Mouse

同种型

IgG2a,κ

背景介绍

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

细胞定位

Cytoplasm. Nucleus. Nucleus membrane. Note=Cytoplasmic when non-complexed Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus.

功能

期货

现货

BD-PB5035 staining CDK4 in human breast carcinoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded Human Breast cancer section using Pink1. BD-PB5035 was diluted at 1:300 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes : Anti-CDK4 Antibody at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.