BD-PB5006-FOXO4 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

FOXO4 Rabbit Polyclonal Antibody (Center)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB5006

产品名称

FOXO4 Rabbit Polyclonal Antibody (Center)

别名

Forkhead box protein O4, Fork head domain transcription factor AFX1, FOXO4, AFX, AFX1, MLLT7

类别

常规抗体

推荐应用

反应种属

Human, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

P98177

特异性

This FOXO4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 206-240 amino acids from the Central region of human FOXO4.

稀释度

IF~~1:25;WB~~1:1000-1:2000

预测分子量

54kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

组织表达

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas

细胞定位

Cytoplasm. Nucleus. Note=When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm

功能

期货

现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling FOXO4 with BD-PB5006 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

All lanes : Anti-FOXO4 Antibody (Center) at 1:1000-1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: K562 whole cell lysates Lane 3: Ramos whole cell lysates Lane 4: NCI-H1299 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.