BD-PB4994-MAML2 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

MAML2 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB4994

产品名称

MAML2 Rabbit Polyclonal Antibody (Center)

别名

Mastermind-like protein 2, Mam-2, MAML2, KIAA1819

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

Q8IZL2

特异性

This MAML2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 780-814 amino acids from the Central region of human MAML2.

稀释度

IHC-P~~1:100~500;WB~~1:2000

预测分子量

125kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.

组织表达

Widely expressed with high levels detected in placenta, salivary gland and skeletal muscle

细胞定位

Nucleus speckle. Note=Nuclear, in a punctate manner

功能

期货

现货

BD-PB4994 staining MAML2 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-MAML2 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 125 kDa Blocking/Dilution buffer: 5% NFDM/TBST.