BD-PB4964 staining DPYSL5 in human tonsil sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-DPYSL5 Antibody (C-term) at 1:2000 dilution Lane 1: Y79 whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lane 3: Raji whole cell lysates Lane 4: mouse brain lysates Lane 5: rat brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 61 kDa Blocking/Dilution buffer: 5% NFDM/TBST.