BD-PB4957-ATP2B3 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ATP2B3 Rabbit Polyclonal Antibody (C-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB4957

产品名称

ATP2B3 Rabbit Polyclonal Antibody (C-term)

类别

常规抗体

推荐应用

反应种属

Human, Rat

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

Q16720

特异性

This ATP2B3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1161-1194 amino acids from the C-terminal region of human ATP2B3.

稀释度

IF~~1:25;WB~~1:2000

预测分子量

134kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

组织表达

Highly expressed in the cerebellum (PubMed:8187550). Expressed in adrenal glands (PubMed:27035656)

细胞定位

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, presynaptic cell membrane {ECO:0000250|UniProtKB:Q64568}; Multi-pass membrane protein. Note=Localized at parallel fiber terminals {ECO:0000250|UniProtKB:Q64568}

功能

ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels at the presynaptic terminals (PubMed:25953895, PubMed:27035656, PubMed:22912398, PubMed:18029012). Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment (PubMed:25953895, PubMed:27035656). May counter-transport protons, but the mechanism and the stoichiometry of this Ca(2+)/H(+) exchange remains to be established (By similarity).

期货

现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling ATP2B3 with BD-PB4957 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on Hela cell line.The nuclear counter stain is DAPI (blue).

Anti-ATP2B3 Antibody (C-term)at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST.