BD-PB4936-SNCA Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

SNCA Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4936

产品名称

SNCA Rabbit Polyclonal Antibody (C-term)

别名

Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse, Cynomolgus Monkey

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

P37840

特异性

This SNCA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-125 amino acids from the C-terminal region of human SNCA.

稀释度

IHC~~1:100~500;IHC-P~~1:100~500;FC~~1:25;WB~~1:1000

预测分子量

14kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

组织表达

Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain

细胞定位

Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Secreted Note=Membrane-bound in dopaminergic neurons

功能

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).

期货

现货

BD-PB4936 staining SNCA in Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

BD-PB4936 staining SNCA in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Flow cytometric analysis of HeLa cells using SNCA Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). BD-PB4936 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysates from Hela cell line, human brain, human cerebellum, mouse brain, rat brain tissue lysate(from left to right), using SNCA Antibody (C-term)at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.