BD-PB4914-EWSR1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

EWSR1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4914

产品名称

EWSR1 Rabbit Polyclonal Antibody (C-term)

别名

RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein, EWSR1, EWS

类别

常规抗体

推荐应用

反应种属

Human, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

Q01844

特异性

This EWSR1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 639-674 amino acids from the C-terminal region of human EWSR1.

稀释度

IF~~1:25;WB~~1:1000

预测分子量

68kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Might normally function as a transcriptionnal repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

组织表达

Ubiquitous.

细胞定位

Nucleus. Cytoplasm. Cell membrane. Note=Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation

功能

Might normally function as a transcriptional repressor. EWS- fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.

期货

现货

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling EWSR1 with BD-PB4914 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1/100 dilution (red).

All lanes : Anti-EWSR1 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysates Lane 2: K562 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.