BD-PB4905-PPP2R5B Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

PPP2R5B Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4905

产品名称

PPP2R5B Rabbit Polyclonal Antibody (C-term)

别名

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, PP2A B subunit isoform B'-beta, PP2A B subunit isoform B56-beta, PP2A B subunit isoform PR61-beta, PP2A B subunit isoform R5-beta, PPP2R5B

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

Q15173

特异性

This PPP2R5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 466-500 amino acids from the C-terminal region of human PPP2R5B.

稀释度

IF~~1:25;WB~~1:1000

预测分子量

57kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.

组织表达

Highest expression in brain.

细胞定位

Cytoplasm.

功能

As the regulatory component of the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme, modulates substrate specificity, subcellular localization, and responsiveness to phosphorylation. The phosphorylated form mediates the interaction between PP2A and AKT1, leading to AKT1 dephosphorylation.

期货

现货

Fluorescent image of SH-SY5Y cells stained with PPP2R5B Antibody (C-term). BD-PB4905 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Western blot analysis of lysate from SK-N-MC cell line, using PPP2R5B Antibody (C-term)at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.