BD-PB4808-SMAD1 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

SMAD1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB4808

产品名称

SMAD1 Rabbit Polyclonal Antibody (Center)

别名

Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, SMAD 1, Smad1, hSMAD1, Transforming growth factor-beta-signaling protein 1, BSP-1, SMAD1, BSP1, MADH1, MADR1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

Q15797

特异性

This SMAD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-196 amino acids from the Central region of human SMAD1.

稀释度

IHC-P~~1:100~500;IF~~1:25;WB~~1:1000

预测分子量

52kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

组织表达

Ubiquitous. Highest expression seen in the heart and skeletal muscle

细胞定位

Cytoplasm. Nucleus Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity) {ECO:0000250|UniProtKB:P70340, ECO:0000269|PubMed:15647271}

功能

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor- regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)- mediated cardiac-specific gene expression.

期货

现货

Immunohistochemical analysis of paraffin-embedded H. heart section using SMAD1 Antibody (Center). BD-PB4808 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of MCF-7 cells stained with SMAD1 Antibody (Center). BD-PB4808 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SMAD1 Antibody (Center). BD-PB4808 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HT-1080, HUVEC, mouse C2C12 cell line and rat liver tissue lysate(from left to right), using SMAD1 Antibody (Center)at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.