BD-PB4801-DFFB Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

DFFB Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB4801

产品名称

DFFB Rabbit Polyclonal Antibody (Center)

别名

DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Swissprot No.

O76075

特异性

This DFFB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from the Central region of human DFFB.

稀释度

IHC-P~~1:100~500;FC~~1:25;WB~~1:1000

预测分子量

39kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

细胞定位

Cytoplasm. Nucleus.

功能

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

期货

现货

Immunohistochemical analysis of paraffin-embedded M.kidney section using DFFB Antibody (Center). BD-PB4801 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.kidney section using DFFB Antibody (Center). BD-PB4801 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of Hela cells using DFFB Antibody (Center)(green) compared to an isotype control of rabbit IgG(blue). BD-PB4801 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysate from A431 cell line, using DFFB Antibody (Center)at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.