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RPS6 (15Y4) Mouse Monoclonal Antibody (N-term)

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产品基本信息

产品货号
BD-PB4799
产品名称
RPS6 (15Y4) Mouse Monoclonal Antibody (N-term)
别名
40S ribosomal protein S6, Phosphoprotein NP33, RPS6
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
存储缓冲液
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.
Human Swissprot No.
P62753
特异性
This RPS6 antibody is generated from a mouse immunized with RPS6 recombinant protein.
稀释度
FC~~1:25;IF~~1:25;IHC-P~~1:100~500;WB~~1:2000
预测分子量
29kDa
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
宿主
Mouse
同种型
IgG1
背景介绍
May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
功能
Component of the 40S small ribosomal subunit (PubMed:8706699). Plays an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA (PubMed:17220279).
期货
现货

Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). BD-PB4799 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term)at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

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