RPS6 (15Y4) Mouse Monoclonal Antibody (N-term)
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Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). BD-PB4799 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). BD-PB4799 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term)at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
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