BD-PB4736-UCHL1 (15T13) Mouse Monoclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

UCHL1 (15T13) Mouse Monoclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4736

产品名称

UCHL1 (15T13) Mouse Monoclonal Antibody (C-term)

别名

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.

Human Gene ID

NP_004172.2

Human Swissprot No.

P09936

特异性

This UCHL1 Monoclonal antibody is generated from mouses immunized with a KLH conjugated synthetic peptide selected from the 185-214 region of human UCHL1.

稀释度

IHC-P~~1:100~500;WB~~1:1000

预测分子量

25kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Mouse

同种型

IgG1

背景介绍

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

组织表达

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

细胞定位

Cytoplasm. Endoplasmic reticulum membrane; Lipid- anchor. Note=About 30% of total UCHL1 is associated with membranes in brain

功能

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865).

期货

现货

Immunohistochemical analysis of paraffin-embedded H. brain section using UCHL1 Antibody (C-term). BD-PB4736 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. astroglioma section using UCHL1 Antibody (C-term). BD-PB4736 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

UCHL1 Antibody (C-term)western blot analysis in 293T,NCI-H1299,rat PC-12 cell line lysates (35μg/lane).This demonstrates the UCHL1 antibody detected the UCHL1 protein (arrow).