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PITPNA Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号
BD-PB4704
产品名称
PITPNA Rabbit Polyclonal Antibody (C-term)
别名
Phosphatidylinositol transfer protein alpha isoform, PI-TP-alpha, PtdIns transfer protein alpha, PtdInsTP alpha, PITPNA, PITPN
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
Human Gene ID
NP_006215.1
Human Swissprot No.
Q00169
特异性
This PITPNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the C-terminal region of human PITPNA.
稀释度
WB~~1:1000;IHC-P~~1:100~500
预测分子量
32kDa
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
宿主
Rabbit
同种型
Rabbit IgG
背景介绍
Catalyzes the transfer of PtdIns and phosphatidylcholine between membranes.
细胞定位
Cytoplasm {ECO:0000250|UniProtKB:P53810}. Nucleus {ECO:0000250|UniProtKB:P53810}
功能
Catalyzes the transfer of phosphatidylinositol (PI) and phosphatidylcholine (PC) between membranes (PubMed:10531358, PubMed:22822086, PubMed:15522822, PubMed:18636990, PubMed:14962392). Shows a preference for PI and PC containing shorter saturated or monosaturated acyl chains at the sn-1 and sn-2 positions (PubMed:15522822, PubMed:22822086). Preference order for PC is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 and for PI is C16:1 > C16:0 > C18:1 > C18:0 > C20:4 > C20:3 (PubMed:22822086).
期货
现货

All lanes : Anti-PITPNA Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: U-251MG whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse brain lysate Lane 7: Rat brain lysate Lysates/proteins at 15 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-PITPNA Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: mouse brain lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: rat brain lysate Lane 7: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded H. skin section using PITPNA Antibody (C-term). BD-PB4704 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

PITPNA Antibody (C-term) western blot analysis in 293,U251,Hela,mouse NIH/3T3 and rat PC12 cell line and mouse brain cerebellum,rat cerebellum and brain lysates (35ug/lane).This demonstrates the PITPNA antibody detected the PITPNA protein (arrow).

PITPNA Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PITPNA antibody detected the PITPNA protein (arrow).

PITPNA Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the PITPNA antibody detected the PITPNA protein (arrow).

PITPNA Antibody (C-term) western blot analysis in rat cerebellum tissue lysates (35ug/lane).This demonstrates the PITPNA antibody detected the PITPNA protein (arrow).

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