BD-PB4702-H2AFX (1R3) Mouse Monoclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

H2AFX (1R3) Mouse Monoclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4702

产品名称

H2AFX (1R3) Mouse Monoclonal Antibody (C-term)

别名

Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX

类别

常规抗体

推荐应用

反应种属

Human, Mouse

存储缓冲液

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein G column, followed by dialysis against PBS.

Human Gene ID

NP_002096.1

Human Swissprot No.

P16104

特异性

This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.

稀释度

IHC-P~~1:100~500;WB~~1:1000;IHC~~1:100~500

预测分子量

15kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Mouse

同种型

IgG1

背景介绍

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

细胞定位

Nucleus. Chromosome

功能

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

期货

现货

Immunohistochemical analysis of paraffin-embedded H. prostate section using H2AFX Antibody (C-term). BD-PB4702 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. thymus section using H2AFX Antibody (C-term). BD-PB4702 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line(from left to right). using H2AFX Antibody (C-term)at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

Immunohistochemical analysis of paraffin-embedded Human Thymus section using Pink1. BD-PB4702 was diluted at 1:50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.