BD-PB4673-TBX1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

TBX1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4673

产品名称

TBX1 Rabbit Polyclonal Antibody (C-term)

别名

T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein, TBX1

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_005983.1;NP_542377.1;NP_542378.1

Human Swissprot No.

O43435

特异性

This TBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-356 amino acids from the C-terminal region of human TBX1.

稀释度

IHC-P~~1:100~500;WB~~1:1000;IF~~1:10~50

预测分子量

43kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.

细胞定位

Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201}.

功能

Probable transcriptional regulator involved in developmental processes (By similarity). Binds to the palindromic T site 5'- TTCACACCTAGGTGTGAA-3' DNA sequence (PubMed:11111039). Is required for normal development of the pharyngeal arch arteries (By similarity).

期货

现货

Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody (C-term). BD-PB4673 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody (C-term) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

TBX1 Antibody (C-term) western blot analysis in HLVEC cell line lysates (35ug/lane).This demonstrates the TBX1 antibody detected the TBX1 protein (arrow).

Fluorescent confocal image of A2058 cell stained with TBX1 Antibody (C-term)was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX1 immunoreactivity is localized to nucleus significantly.

Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.