BD-PB4658-OAT Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

OAT Rabbit Polyclonal Antibody (N-term)

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产品基本信息

产品货号

BD-PB4658

产品名称

OAT Rabbit Polyclonal Antibody (N-term)

别名

Ornithine aminotransferase, mitochondrial, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, Ornithine aminotransferase, hepatic form, Ornithine aminotransferase, renal form, OAT

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_000265.1;NP_001165285.1

Human Swissprot No.

P04181

特异性

This OAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human OAT.

稀释度

WB~~1:1000;IF~~1:10~50;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

49kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.

细胞定位

Mitochondrion matrix

功能

Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.

期货

现货

All lanes : Anti-OAT Antibody (N-term) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

OAT Antibody (N-term) western blot analysis in 293,293T cell line ,mouse liver and rat liver tissue lysates (35ug/lane).This demonstrates the OAT antibody detected the OAT protein (arrow).

Fluorescent image of A549 cell stained with OAT Antibody (N-term)was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).OAT immunoreactivity is localized to Mitochondrion significantly.

Formalin-fixed and paraffin-embedded human brain tissue reacted with OAT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

OAT Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.