产品名称
VATA Rabbit Polyclonal Antibody
基因名称
ATP6V1A ATP6A1 ATP6V1A1 VPP2
蛋白名称
V-type proton ATPase catalytic subunit A (V-ATPase subunit A) (EC 3.6.3.14) (V-ATPase 69 kDa subunit) (Vacuolar ATPase isoform VA68) (Vacuolar proton pump subunit alpha)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P38606
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P38606/entry
Mouse Swissprot No.
P50516
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P50516
免疫原
Synthesized peptide derived from part region of human protein
特异性
VATA Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all
组织表达
High expression in the skin.
细胞定位
Cytoplasm . Cytoplasm, cytosol . Cytoplasmic vesicle, secretory vesicle . Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein . Lysosome . Co-localizes with WFS1 in the secretory granules in neuroblastoma cell lines. .
信号通路
Oxidative phosphorylation;Vibrio cholerae infection;Epithelial cell signaling in Helicobacter pylori infection;
功能
catalytic activity:ATP + H(2)O + H(+)(In) = ADP + phosphate + H(+)(Out).,function:Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.,similarity:Belongs to the ATPase alpha/beta chains family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).,tissue specificity:Present in all tissues analyzed.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
