BD-PB4648-EHHADH Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

EHHADH Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4648

产品名称

EHHADH Rabbit Polyclonal Antibody (C-term)

别名

Peroxisomal bifunctional enzyme, PBE, PBFE, Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase, 3-hydroxyacyl-CoA dehydrogenase, EHHADH, ECHD

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001159887.1;NP_001957.2

Human Swissprot No.

Q08426

特异性

This EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.

稀释度

WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

79kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity.

组织表达

Liver and kidney. Strongly expressed in the terminal segments of the proximal tubule. Lower amounts seen in the brain.

细胞定位

Peroxisome.

功能

Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl- CoA isomerase activities. Catalyzes two of the four reactions of the long straight chain fatty acids peroxisomal beta-oxidation pathway. Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3- cis and 3-trans double bonds into the 2-trans form in a range of enoyl- CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (PubMed:15060085).

期货

现货

Western blot analysis of lysates from mousr kidney, rat kidney and liver tissue (from left to right), using EHHADH Antibody (C-term)at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot analysis of EHHADH Antibody (C-term) in mouse liver(lane 1), kidney(lane 2) tissue lysates (35ug/lane). EHHADH (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of HepG2 cells using EHHADH Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.