BD-PB4646-BCL2L11 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

BCL2L11 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB4646

产品名称

BCL2L11 Rabbit Polyclonal Antibody (Center)

别名

Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001191035.1;NP_001191036.1;NP_001191037.1;NP_001191038.1;NP_001191039.1;NP_001191040.1;NP_001191041.1;NP_001191042.1;NP_006529.1;NP_619527.1;NP_619528.1;NP_619529.1;NP_619530.1;NP_619531.1;NP_61953

Human Swissprot No.

O43521

特异性

This BCL2L11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human BCL2L11.

稀释度

IHC-P~~1:100~500;WB~~1:1000;FC~~1:10~50

预测分子量

22kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

BCL2L11 belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator.

组织表达

Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.

细胞定位

Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. [Isoform BimL]: Mitochondrion. [Isoform Bim-alpha1]: Mitochondrion.

功能

Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase- mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.

期货

现货

Immunohistochemical analysis of paraffin-embedded H. kidney section using BCL2L11 Antibody (Center). BD-PB4646 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of BCL2L11 Antibody (Center) in K562, HL-60 cell line lysates (35ug/lane). BCL2L11 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with BCL2L11 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometric analysis of WiDr cells using BCL2L11 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.