BD-PB4609-ACAT1 Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

ACAT1 Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4609

产品名称

ACAT1 Rabbit Polyclonal Antibody (C-term)

别名

Acetyl-CoA acetyltransferase, mitochondrial, Acetoacetyl-CoA thiolase, T2, ACAT1, ACAT, MAT

类别

常规抗体

推荐应用

反应种属

Human, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_000010.1

Human Swissprot No.

P24752

特异性

This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

45kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

细胞定位

Mitochondrion.

功能

This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA (PubMed:1715688, PubMed:7728148, PubMed:9744475). Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms (PubMed:1715688, PubMed:7728148, PubMed:9744475). The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA (PubMed:17371050). Thereby, it plays a major role in ketone body metabolism (PubMed:17371050, PubMed:1715688, PubMed:7728148, PubMed:9744475).

期货

现货

All lanes : Anti-ACAT1 Antibody (C-term) at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution Lane 1: SK-BR-3 whole cell lysates Lane 2: HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45. 2 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of anti-ACAT1 Pab in SK-BR-3 cell line lysates (35ug/lane).ACAT1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.