BD-PB4603-IDH1 Rabbit Polyclonal Antibody (Center)-现货抗体产品库武汉枢密脑科学技术有限公司

IDH1 Rabbit Polyclonal Antibody (Center)

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产品基本信息

产品货号

BD-PB4603

产品名称

IDH1 Rabbit Polyclonal Antibody (Center)

别名

Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, IDH1, PICD

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_001269315.1;NP_001269316.1;NP_005887.2

Human Swissprot No.

O75874

特异性

This IDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the Central region of human IDH1.

稀释度

IHC-P~~1:100~500;WB~~1:1000;IF~~1:10~50;FC~~1:10~50

预测分子量

47kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

细胞定位

Cytoplasm, cytosol. Peroxisome

期货

现货

Immunohistochemical analysis of paraffin-embedded H. prostate section using IDH1 Antibody (Center). BD-PB4603 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right), using IDH1 Antibody (Center)at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Confocal immunofluorescent analysis of IDH1 Antibody (Center)with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDH1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of IDH1 Antibody (Center) in HepG2 cell line and mouse liver tissue lysates (35ug/lane). IDH1 (arrow) was detected using the purified Pab.

IDH1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.