BD-PB4595-GSN Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GSN Rabbit Polyclonal Antibody (C-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB4595

产品名称

GSN Rabbit Polyclonal Antibody (C-term)

别名

Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin, GSN

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_000168.1;NP_001121134.1;NP_001121135.2;NP_001121136.1;NP_001121137.1;NP_001121138.1;NP_001121139.1;NP_001244958.1;NP_001244959.1;NP_937895.1

Human Swissprot No.

P06396

特异性

This GSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714-743 amino acids from the C-terminal region of human GSN.

稀释度

WB~~1:1000-1:2000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

86kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

GSN binds to the 'plus' ends of actin monomers and filaments to prevent monomer exchange. The calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this protein are a cause of familial amyloidosis Finnish type (FAF).

组织表达

Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells

细胞定位

[Isoform 2]: Cytoplasm, cytoskeleton.

功能

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.

期货

现货

All lanes : Anti-GSN Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: mouse spleen lysates Lane 3: MCF-7 whole cell lysates Lane 4: human plasma lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

GSN Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSN Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GSN Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.