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NPTX1-Y344 Rabbit Polyclonal Antibody

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产品基本信息

产品货号
BD-PB4590
产品名称
NPTX1-Y344 Rabbit Polyclonal Antibody
别名
Neuronal pentraxin-1, NP1, Neuronal pentraxin I, NP-I, NPTX1
类别
常规抗体
推荐应用
WB
反应种属
Human, Rat, Mouse
存储缓冲液
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.
Human Gene ID
NP_002513.2
Human Swissprot No.
Q15818
特异性
This NPTX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from human NPTX1.
稀释度
IHC-P~~1:100~500;WB~~1:2000
预测分子量
47kDa
运输及保存条件
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
宿主
Rabbit
同种型
Rabbit IgG
背景介绍
NPTX1 is a member of the neuronal pentraxin family. Human neuronal pentraxin 1 is similar to rat NP1 which is a binding protein for the snake venom toxin taipoxin.
细胞定位
Cytoplasmic vesicle, secretory vesicle
功能
May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
期货
现货

BD-PB4590 staining NPTX1 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes : Anti-NPTX1Y344 Antibody at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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