BD-PB4579-MOB4A Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

MOB4A Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4579

产品名称

MOB4A Rabbit Polyclonal Antibody (C-term)

别名

MOB kinase activator 1B, Mob1 homolog 1A, Mob1A, Mob1B, Mps one binder kinase activator-like 1A, MOB1B, MOB4A, MOBKL1A

类别

常规抗体

推荐应用

反应种属

Human, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_001231695.1;NP_775739.1

Human Swissprot No.

Q7L9L4

特异性

This MOB4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-206 amino acids from the C-terminal region of human MOB4A.

稀释度

WB~~1:1000;IF~~1:10~50;IHC-P~~1:100~500

预测分子量

25kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

MOB4A is similar to the yeast Mob1 protein. Yeast Mob1 binds Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation. Mob4A regulates NDR1 and NDR2 kinase activity, widely expressed nuclear serine-threonine kinases that have been implicated in cell proliferation and/or tumor progression.

组织表达

Adrenal gland, bone marrow, brain, lung, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, uterus, colon with mucosa, fetal brain and fetal liver

细胞定位

Cytoplasm. Nucleus

功能

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.

期货

现货

Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

MOB4A Antibody (C-term) western blot analysis in Daudi,K562 cell line lysates (35ug/lane).This demonstrates the MOB4A antibody detected the MOB4A protein (arrow).

Fluorescent image of NIH/3T3 cell stained with MOB4A Antibody (C-term)was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).MOB4A immunoreactivity is localized to Cytoplasm significantly.

Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.