BD-PB4564-GTF2I Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

GTF2I Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4564

产品名称

GTF2I Rabbit Polyclonal Antibody (C-term)

别名

General transcription factor II-I, GTFII-I, TFII-I, Bruton tyrosine kinase-associated protein 135, BAP-135, BTK-associated protein 135, SRF-Phox1-interacting protein, SPIN, Williams-Beuren syndrome chromosomal region 6 protein, GTF2I, BAP135, WBSCR6

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001157108.1;NP_001267729.1;NP_001509.3;NP_127492.1;NP_127493.1;NP_127494.1

Human Swissprot No.

P78347

特异性

This GTF2I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 956-985 amino acids from the C-terminal region of human GTF2I.

稀释度

IF~~1:10~50;WB~~1:1000;IHC-P~~1:100~500;FC~~1:10~50

预测分子量

112kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

GTF2I is a multifunctional phosphoprotein with roles in transcription and signal transduction. It is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23.

组织表达

Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues

细胞定位

Cytoplasm. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00484, ECO:0000269|PubMed:10373551} Note=Colocalizes with BTK in the cytoplasm

功能

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA- binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.

期货

现货

Fluorescent image of Hela cells stained with GTF2I Antibody (C-term). BD-PB4564 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

Western blot analysis of lysates from A431, Hela cell line (from left to right), using GTF2I Antibody (C-term)at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Fluorescent image of A549 cell stained with GTF2I Antibody (C-term)was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).GTF2I immunoreactivity is localized to Nucleus significantly.

GTF2I Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the GTF2I antibody detected the GTF2I protein (arrow).

Western blot analysis of GTF2I Antibody (C-term) in Y79 cell line lysates (35ug/lane). GTF2I (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with GTF2I Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

GTF2I Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.