Annexin V Rabbit Polyclonal Antibody (N-term)
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue using BD-PB4554 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
All lanes : Anti-Annexin V Antibody (N-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: A2058 whole cell lysate Lane 5: T47D whole cell lysate Lane 6: Mouse lung lysate Lane 7: Mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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