BD-PB4548-GRB2 Rabbit Polyclonal Antibody (Y209)-现货抗体产品库武汉枢密脑科学技术有限公司

GRB2 Rabbit Polyclonal Antibody (Y209)

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产品基本信息

产品货号

BD-PB4548

产品名称

GRB2 Rabbit Polyclonal Antibody (Y209)

别名

Growth factor receptor-bound protein 2, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2, GRB2, ASH

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_002077.1;NP_987102.1

Human Swissprot No.

P62993

特异性

This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from human GRB2.

稀释度

WB~~1:1000;IHC-P~~1:100~500

预测分子量

25kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway.

细胞定位

Nucleus. Cytoplasm. Endosome Golgi apparatus {ECO:0000250|UniProtKB:Q60631}

功能

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

期货

现货

Western blot analysis of lysate from Ramos cell line, using GRB2 Antibody (pY209)at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 Antibody (Y209), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.