BD-PB4545-Anterior Gradient 2 (AGR2) Rabbit Polyclonal Antibody (N-term)-现货抗体产品库武汉枢密脑科学技术有限公司

Anterior Gradient 2 (AGR2) Rabbit Polyclonal Antibody (N-term)

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PB4545

产品名称

Anterior Gradient 2 (AGR2) Rabbit Polyclonal Antibody (N-term)

别名

Anterior gradient protein 2 homolog, AG-2, hAG-2, HPC8, Secreted cement gland protein XAG-2 homolog, AGR2, AG2

类别

常规抗体

推荐应用

反应种属

Human, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Human Gene ID

NP_006399.1

Human Swissprot No.

O95994

特异性

This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).

稀释度

WB~~1:1000;IF~~1:10~50;IHC-P~~1:100~500

预测分子量

20kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Anterior gradient 2 (AGR2) is known as a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. AGR2 has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells (CTCs). Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells, but conditions leading to increased expression of AGR2 are not well understood.

组织表达

Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines

细胞定位

Secreted. Endoplasmic reticulum

功能

Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion (PubMed:23274113).

期货

现货

AGR2 Antibody (N-term) western blot analysis in HT29,MCF-7,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow).

Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term)was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.