BD-PB4543-p53 Rabbit Polyclonal Antibody (S315)-现货抗体产品库武汉枢密脑科学技术有限公司

p53 Rabbit Polyclonal Antibody (S315)

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产品基本信息

产品货号

BD-PB4543

产品名称

p53 Rabbit Polyclonal Antibody (S315)

别名

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

类别

常规抗体

推荐应用

反应种属

Human

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_000537.3;NP_001119584.1;NP_001119585.1;NP_001119586.1;NP_001119587.1;NP_001119588.1;NP_001119589.1;NP_001119590.1;NP_001263624.1;NP_001263625.1;NP_001263626.1;NP_001263627.1;NP_001263628.1;NP_00126

Human Swissprot No.

P04637

特异性

This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-322 amino acids from human p53.

稀释度

WB~~1:1000;IHC-P~~1:100~500;IF~~1:10~50

预测分子量

0kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

期货

现货

All lanes : Anti-p53 Antibody (S315) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

p53 Antibody (S315) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the p53 antibody detected the p53 protein (arrow).

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with p53 Antibody (S315) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent confocal image of U251 cell stained with p53 Antibody (S315)was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). p53 immunoreactivity is localized to Nucleus significantly.