BD-PB4533-CALCR Rabbit Polyclonal Antibody (C-term)-现货抗体产品库武汉枢密脑科学技术有限公司

CALCR Rabbit Polyclonal Antibody (C-term)

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产品基本信息

产品货号

BD-PB4533

产品名称

CALCR Rabbit Polyclonal Antibody (C-term)

别名

Calcitonin receptor, CT-R, CALCR

类别

常规抗体

推荐应用

反应种属

Human, Rat, Mouse

存储缓冲液

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) New type preservative N. This antibody is purified through a protein A column, followed by peptide affinity purification.

Human Gene ID

NP_001158209.1;NP_001158210.1;NP_001733.1

Human Swissprot No.

P30988

特异性

This CALCR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-494 amino acids of human CALCR.

稀释度

WB~~1:1000;FC~~1:10~50;IF~~1:10~50

预测分子量

55kDa

运输及保存条件

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

宿主

Rabbit

同种型

Rabbit IgG

背景介绍

CALCR is a high affinity receptor for the peptide hormone calcitonin and belongs to a subfamily of seven transmembrane-spanning G protein-coupled receptors. The encoded protein is involved in maintaining calcium homeostasis and in regulating osteoclast-mediated bone resorption. Polymorphisms in this gene have been associated with variations in bone mineral density and onset of osteoporosis.

细胞定位

Cell membrane; Multi-pass membrane protein.

功能

This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.

期货

现货

Western blot analysis of lysate from MCF-7, A431 cell line, using CALCR Antibody (C-term)with(+) or without(-) peptides. BD-PB4533 was diluted at 1:1000 at each lane. A goat anti-rabbit(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

CALCR Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of CALCR Antibody (C-term)with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).