产品名称
UB2E3 Rabbit Polyclonal Antibody
蛋白名称
Ubiquitin-conjugating enzyme E2 E3 (EC 6.3.2.19) (UbcH9) (Ubiquitin carrier protein E3) (Ubiquitin-conjugating enzyme E2-23 kDa) (Ubiquitin-protein ligase E3)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q969T4
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q969T4/entry
Mouse Swissprot No.
P52483
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P52483
免疫原
Synthesized peptide derived from part region of human protein
特异性
UB2E3 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013],
组织表达
Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.
细胞定位
Nucleus . Cytoplasm . Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner.
信号通路
Ubiquitin mediated proteolysis;
功能
catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subcellular location:Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner.,subunit:The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus (By similarity). Interacts with NEDD4L.,tissue specificity:Ubiquitously expressed at low levels. Highly expressed in skeletal muscle.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
